Fig. 4. Effect of HT on Ca²⁺ sensitivity of LPA-induced PS exposure in RBC: Cells were treated with LPA for 24 h in the absence and presence of increasing concentrations of HT both in absence or presence of Ca²⁺ . B, C. Original histogram of annexin-V-binding in RBC. A. Arithmetic means ± SEM (n = 8) of annexin-V-binding of RBC. Statistical analysis was performed with one-way ANOVA followed by Tukey's test. * (p<0.05) and ** (p<0.01) indicate significant difference from the absence of LPA. # (p<0.05) and ## (p<0.01) indicate significant differences from cells lacking HT treatment. ++ (p<0.05) indicates significant difference from corresponding value in the presence of Ca²⁺ .